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From Soybeans to Tofu: The Underlying Chemistry

From Soybeans to Tofu: The Underlying Chemistry
graphical abstract for the article showing images progressing from soy to tofu and a chemical representation of protein denaturation and coagulation
Summary
Tofu, a traditional Chinese food, is widely consumed around the world, yet the chemistry behind its production is often overlooked. This study presents a simple demonstration designed for lower-level undergraduate courses in organic chemistry or biochemistry to elucidate the key principles involved in the tofu-making process. The research highlights the role of heat treatment in denaturing soy proteins, which facilitates coagulation when coagulants such as calcium gluconate, zinc gluconate, and calcium lactate are added. Acidic coagulants, including white vinegar and citrus juices, also induce coagulation due to their impact on the isoelectric point of soy proteins. The study provides a detailed analysis of the curdling mechanism based on experimental data and previous research, making it an effective educational tool for both laboratory and at-home learning environments.

Summary:
Authors/Contributors: Bingxing Wang, Qi Wang, Bingli Wang, Songlin Wang, Yongcai Zhang, and Donglin Zhao.

Citation: Wang, B., Wang, Q., Wang, B., Wang, S., Zhang, Y., & Zhao, D. (2023). From Soybeans to Tofu: The Underlying Chemistry. Journal of Chemical Education, 100, 3724–3730. https://doi.org/10.1021/acs.jchemed.3c00096

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Moderation state
Published
Object Type
Journal articles
Audience
Introductory Undergraduate
Published on
Green Chemistry Principles
Use of Renewable Feedstocks
U.N. Sustainable Development Goals (SDGs)
Responsible Consumption and Production
Climate Action
Learning Goals/Student Objectives
This learning activity aims to enhance students' understanding of the fundamental chemistry principles involved in tofu production while promoting green chemistry practices. Students will explore the concepts of protein denaturation, coagulation mechanisms, and the role of acids and salts as coagulants in a sustainable food-processing context.

By the end of this activity, students should be able to:

1. Explain Protein Chemistry
a. Describe the structure of soy proteins and how heat treatment leads to their denaturation.
b. Define the isoelectric point and its significance in protein coagulation.

2. Understand the Chemistry of Coagulation
a. Compare the effects of different coagulants (salts vs. acids) on soy protein aggregation.
b. Analyze how pH influences the formation of tofu and other protein-based food products.

3. Apply Green Chemistry Principles
a. Identify how tofu production aligns with green chemistry concepts, including the use of renewable resources and minimizing waste.
b. Evaluate the environmental impact of traditional and alternative coagulants.

4. Encourage Sustainable Food Science Innovations
a. Discuss how chemistry can be used to develop plant-based food alternatives.
b. Explore opportunities for improving tofu production through innovative, eco-friendly methods.
Common pedagogies covered
Context-based learning

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Safety Precautions, Hazards, and Risk Assessment
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Teacher Recommendations or Piloting Data (if available)
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